dctP [2019-02-19 18:23:39]

uptake of succinate, fumurate, malate and oxaloacetate via proton symport

locus: BSU04470

pI: 8.67

mw: 45.28 kDa

protein length: 421 aa

gene length: 1266 bp

function: uptake of succinate, fumurate, malate and oxaloacetate

product: C4-dicarboxylate transport protein

essential: no

synonyms: ydbH,dctA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.6|Dicarboxylate/amino acid:cation symporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 500,166 501,431

The protein

Catalyzed reaction/ biological activity

• uptake of succinate, fumurate, malate and oxaloacetate via proton symport [Pubmed|20363944]
• acts as co-sensor for [protein|9A96572B101358768EB20EE9438BE0E1424A4130|DctS] [Pubmed|24375102]

Protein family

• [SW|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)

Paralogous protein(s)

• [protein|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|GltT], [protein|622856EE642C42247C658DBD57E6A18C6E81FE58|GltP]

Structure

• [PDB|4KY0] (the glutamate transporter of ''Thermococcus kodakarensis'', 31% identity, 71% similarity) [Pubmed|24013209]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]
  description: [Pubmed|10708364]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10708364], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: indirect effect, in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]

• genes: [gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]-[gene|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]-[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]
  description: [Pubmed|10708364]

  regulation

  • part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]

Other regulations

• [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition, [Pubmed|22389480]

Biological materials

Mutant

• BKE04470 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTTCATCCCCCTGT, downstream forward: _UP4_TGAAAAAAGAACGGCCCATC
• BKK04470 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTTCATCCCCCTGT, downstream forward: _UP4_TGAAAAAAGAACGGCCCATC
• GP2600 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::tet comIQ12L) (in DK1042) available at [SW|Jrg Stlke]'s lab

References

• 12850135,10708364,10627041,12949159,20363944,22389480,22900538,24013209,24375102